Influence of GlycoGag on the Incorporation of Host Membrane Proteins Into the Envelope of the Moloney Murine Leukemia Virus

Analysis of viral particle heterogeneity produced from infected cells has been limited by the inefficiency traditional analytical methods to characterize large populations viruses at an individual level. Flow virometry (FVM) is emerging technique based on flow cytometry principles that enables a high throughput, multiparametric, and phenotypic characterization single resolution. Here, we performed FVM analyze surface markers found Murine Leukemia Virus (MLV) glycosylated Gag-deficient (glycoGag) MLV. The glycoGag accessory protein several roles in MLV infection cycle including directing retroviral assembly release lipid rafts. Based previous studies, hypothesize modulates host incorporation into envelope during budding. using FVM, reveal associated with increased host-derived tetraspanins CD81 CD63 along raft marker immune antigen Thy1.2 particles NIH 3T3, EL4, primary CD4+ T cells. Moreover, show differences uptake proteins are released two cell lines lymphocytes. Additionally, level, observed degree expression antigens within population. Finally, certain cellular can either enrichment or exclusion depending whether expressed virus. This suggests involved mechanism selective envelope.